Files
Publication Date
Spring 2025
Keywords
SOX18, Cancer, DNA, Cells, Ewing Sarcoma
Description
SOX18, a transcription factor with a DNA-binding HMG domain, plays a critical role in regulatory processes linked to cancer progression. Exhibiting oncogenic properties, SOX18 has been linked to various cancers, where it promotes tumor growth by enhancing cell invasion, uncontrolled proliferation, and resistance to apoptosis through dysregulated signaling pathways. STAT1, a protein crucial for immune system regulation, is prominently expressed in immune-associated tissues like the lymph nodes, and bone marrow, suggesting it could play a role in tumor growth. Ewing Sarcoma (EWS), an aggressive cancer that targets bone and soft tissue, carries a high mortality rate of 90% without treatment and 75-85% even with standard therapies, with a substantial risk of relapse. Given the involvement of SOX18 in oncogenesis and the immune-regulatory role of STAT1, we hypothesized that SOX18 expression would result in increased STAT1 and that knockdown of STAT1 would reduce cell viability and increase apoptosis. This would suggest that SOX18 contributes to the survival and proliferation of EWS cells. Furthermore, if SOX18 is overexpressed into the cell line of STAT1, which is associated with necroptosis, it may decrease cell viability and produce more cancerous cells.
Collection
Cell and Molecular Methods
Format
Medium
Lab report, poster
Size or Duration
21 pages
City
San Antonio
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Castillo, Victoria; Cervantes, Melany; Robles, Nicole; and Shackleford, Terry, "Test Tubes" (2025). Cell and Molecular Methods. 2.
https://commons.stmarytx.edu/biostulab25/2
