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Digital Publisher
Digital Commons at St. Mary's University
Publication Date
Spring 2024
Keywords
Bacterium, Mycoplasma Pneumoniae, Respiratory Diseases, Community Acquired Respiratory Distress Syndrome Toxin
Description
Mycoplasma pneumoniae (Mp) is an atypical bacterium that is linked to various respiratory diseases such as walking pneumonia and asthma. Upon infection, Mp produces a 591-aa virulence factor known as Community Acquired Respiratory Distress Syndrome Toxin (CARDS TX). The crystal structure of this 591-aa cytotoxin reveals a triangular molecule comprised of an N-terminal ADP-ribosylating domain and a Cterminal tandem β-trefoil domain that is responsible for vacuolation of the host’s cells. Based on structural and sequence homology to other ADP ribosylating toxins, the NAD+ binding site has been predicted, yet none of the published structures of the CARDS toxin contain NAD+. The loop connecting the N- and the C- terminal domains is anchored using a disulfide bond that surrounds the NAD+ binding site which might sterically hinder NAD+ binding. An N-terminal truncation mutant has been cloned and the protein was expressed and purified in pursuit of the structure of Domain 1 bound to NAD+.
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1 page
City
San Antonio, Texas
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This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
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Other Biochemistry, Biophysics, and Structural Biology Commons, Structural Biology Commons