cifA and cifB expression in response to Piwi and AUB knockdown in JW18 D. melanogaster cells

Authors

Julieta Alarcon, St. Mary's University

Files

Digital Publisher

Digital Commons at St. Mary's University

Publication Date

Spring 2024

Contributor

Gray, Christine (Faculty Mentor)

Keywords

Bacterium, Cytoplasmic Incompatibility, Drosophila Melanogaster, Biological Research, Fruit Flies

Description

Wolbachia is an intracellular bacterium that is known for inducing cytoplasmic incompatibility (CI) in its Drosophila melanogaster hosts. CI is characterized by embryonic death when males that express cifA and cifB genes mate with unaffected females (Shropshire, Rosenberg, and Bordenstein 2020). PIWI proteins play important roles such as germline determination, spermiogenesis, and transposon silencing (Thomson and Lin 2009). Previous studies have shown that Piwi expression decreases in a developmental window called the “Piwiless pocket” or Pilp. When Aub is depleted in the Pilp, then Piwi expression increases (Théron et al. 2018). We will determine if Piwi and Aub FANA ASO knockdowns affect the expression of cifA and cifB.

Format

pdf

Size

1 page

City

San Antonio, Texas

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.