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Publication Date
Summer 2025
Digital Publisher
Digital Commons at St. Mary's University
Collection
McNair Scholars Symposium
Keywords
Phosphatidylethanol (PEth), Blood, Ethanol, Cholesterol, High-density Lipoprotein (HDL), Low-Density Lipoprotein (LDL)
Description
Background: Phosphatidylethanol (PEth) is a metabolite of ethanol, therefore a direct detector of alcohol in the human body, which is used to identify and monitor severity of alcohol events.
There are standardized levels that indicate little to heavy drinking, ranging from 0-200+. PEth levels, however, are variable amongst humans. Non-human primates such as monkeys have been proven to produce PEth, being a viable model to test for these sources of variability amongst humans. In this study, we examined the relationship of cholesterol and lipid proteins to an abundant PEth homolog [16:0/18:01] in non-human primates existing in both humans and monkeys.
Methods: 6 monkeys were given unrestricted access to 4% ethanol solution and water 20 hours/day for 14 days. Whole blood samples were taken afterwards and then analyzed for Peth homolog [16:0/18:01] using high performance liquid chromatography (HPLC) as well as for total cholesterol, HDL, and LDL using an AF HDL & LDL/VLDL assay kit (ELISA).
Results: The results show that 1) PEth to lipid counts have a better accuracy when read immediately after alcohol consumption, compared to after 2 weeks; 2) the PEth/HDL had the highest correlation when reflected in volume intake (amount); 3) PEth/LDL had the highest correlation when reflected in concentration (dose).
Conclusion: Results suggest PEth to lipid ratios have a stronger correlation when blood is tested more recently compared to over time, as well as when PEth is compared to recent alcohol intake alone. More research needs to be conducted to better understand the relationship between phosphatidylethanol and lipid interaction.
Disciplines
Animal Experimentation and Research | Biological Factors | Lipids | Other Chemicals and Drugs | Substance Abuse and Addiction
Format
MOV
Medium
Video
Size or Duration
18 minutes 31 seconds
City
San Antonio, Texas
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
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